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antiphospho tyr1007 jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antiphospho tyr1007 jak2
    Antiphospho Tyr1007 Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antiphospho+jak2/pm41895382-88-63-64
    Average 86 stars, based on 1 article reviews
    antiphospho tyr1007 jak2 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Osteoblast-restricted Disruption of the Growth Hormone Receptor in Mice Results in Sexually Dimorphic Skeletal Phenotypes
    Article Snippet: Human IGF-1 was obtained from GroPep (Theberton, SA, Australia) and stored in 1 000X aliquots for single use. .. Antibodies used for immunoblotting included anti-βactin (C4) from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); antiphospho-JAK2 (Tyr1007/1008), anti-JAK2, antiphospho-STAT5 (Tyr 694), anti-STAT5, anti-phospho-Akt (Ser 473), anti-Akt, anti-phospho-ERK (Thr202/Tyr204), anti-ERK, anti-IGF-1R from Cell Signaling Technology (Danvers, MA), and anti-GHR AL47 (a generous gift from Dr. Stuart Frank, University of Alabama at Birmingham, Birmingham, AL). .. Horseradish-peroxidase conjugated rabbit and mouse secondary antibodies were obtained from Pierce Bio-technology (Rockford, IL).

    other:

    Article Title: Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals
    Article Snippet: Antibodies used in this study include anti-CCNA1 (Sangon biotech, Cat# D220507, Shanghai, China), anti-CCND1 (Sangon biotech, Cat# D220509), anti-CCNE1 (Cell Signaling Technology, Cat# 2080S, Danvers, MA, USA), anti-CDK4 (Sangon biotech, Cat# D120396), anti-PPARa (Abconal, Cat# A18252, Wuhan, China), anti-CPT1a (Proteintech, Cat# 15148-1, Wuhan, China), anti-HMGCS2 (Abconal, Cat# A23545), anti-HMGCL (Sangon biotech, Cat# D122488), anti-BDH1 (Proteintech, Cat# 67448-1), anti-JAK2 (Cell Signaling Technology, Cat# 3230T), antiPhospho-JAK2 (Cell Signaling Technology, Cat# 3771S), antiSTAT3 (Sangon biotech, Cat# D190759), anti-Phospho-STAT3 (Tyr705) (Cell Signaling Technology, Cat# 9145), anti-acetylStat3 (Lys685) (Cell Signaling Technology, Cat# 2523) and antic-Myc (Proteintech, Cat# 67447-1).

    Incubation:

    Article Title: Inhibitory Effects of Compounds and Extracts from Ampelopsis brevipedunculata on IL-6-Induced STAT3 Activation.
    Article Snippet: .. Next, the membranes were incubated overnight at 4∘C with primary antibody, including anti-phospho-STAT3 (1:1000), anti-STAT3 (1:1000), antiphospho-JAK2 (1:1000), anti-JAK2 (1:1000), anti-phosphoERK (1:1000), and anti-ERK (1:1000) antibodies (Cell Signaling, Beverly,MA,USA).Themembraneswere then incubated with an appropriate horseradish peroxide-conjugated secondary antibody (1:5000) at room temperature. ..

    Membrane:

    Article Title: <p>Electroacupuncture treatment upregulates α7nAChR and inhibits JAK2/STAT3 in dorsal root ganglion of rat with spared nerve injury</p>
    Article Snippet: This procedure was performed as described in previous study.31 Thirty micrograms of protein per sample were denatured and then separated with 10% SDS-PAGE and western-blotted on a PVDF (Millipore, CA) membrane using a minigel and mini transblot apparatus (Bio-Rad, Hercules, CA). .. The membrane was blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 for 60 mins at room temperature, and subsequently the membrane respectively immuno-labelled overnight at 4°C with antibodies of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), anti-phospho-STAT3 (Y785) XP® Rabbit mAb (1:200, D3A7, CST, UK), anti-STAT3 mAb (1:400, 124H6, CST, UK), antiphospho-JAK2 (Tyr1007/1008) (1:200, 3771, CST, UK) or anti-JAK2 (D2E12) XP® Rabbit mAb (1:400, 3230, CST, UK), anti-IL-1β (1:200, ab6722, Abcam, UK), antiIL-6 (1:200, ab6722, Abcam, UK), anti-IL-10 (1:200, SC365858, Santa Cruz, UK), β-actin (1:1,000, Solarbio, China) or β-tubulin (1:1,000, Solarbio, China). .. The blots were washed with Tris-buffered saline and Tween and then incubated with the horseradish peroxidase-conjugated antirabbit secondary antibody (1:1,000, Cell Signaling Technology, USA) for 60 mins at 4°C.

    Saline:

    Article Title: <p>Electroacupuncture treatment upregulates α7nAChR and inhibits JAK2/STAT3 in dorsal root ganglion of rat with spared nerve injury</p>
    Article Snippet: This procedure was performed as described in previous study.31 Thirty micrograms of protein per sample were denatured and then separated with 10% SDS-PAGE and western-blotted on a PVDF (Millipore, CA) membrane using a minigel and mini transblot apparatus (Bio-Rad, Hercules, CA). .. The membrane was blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 for 60 mins at room temperature, and subsequently the membrane respectively immuno-labelled overnight at 4°C with antibodies of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), anti-phospho-STAT3 (Y785) XP® Rabbit mAb (1:200, D3A7, CST, UK), anti-STAT3 mAb (1:400, 124H6, CST, UK), antiphospho-JAK2 (Tyr1007/1008) (1:200, 3771, CST, UK) or anti-JAK2 (D2E12) XP® Rabbit mAb (1:400, 3230, CST, UK), anti-IL-1β (1:200, ab6722, Abcam, UK), antiIL-6 (1:200, ab6722, Abcam, UK), anti-IL-10 (1:200, SC365858, Santa Cruz, UK), β-actin (1:1,000, Solarbio, China) or β-tubulin (1:1,000, Solarbio, China). .. The blots were washed with Tris-buffered saline and Tween and then incubated with the horseradish peroxidase-conjugated antirabbit secondary antibody (1:1,000, Cell Signaling Technology, USA) for 60 mins at 4°C.



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